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Emfret清除抗體Antibodies for Mouse Platelet Depletion

更新時間:2023-07-16   點擊次數:686次

德國Emfret公司專注心血管和血液系統研究用抗體研發和生產,供應的抗體包括清除血液中血小板、流式細胞術鑒定分析小鼠血小板表面糖蛋白,emfret公司的抗體不僅適用于流失分析、WB檢測,還可用于免疫組化/免疫熒光及免疫共沉淀檢測。

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背景資料:

This antibody preparation contains a mixture of purified rat monoclonal antibodies directed against mouse GPIb (CD42b). Targeting of this receptor with divalent IgGs results in profound and irreversible Fc-independent platelet depletion in mice1,2 . Platelet depletion is frequently used to study the general role of platelets in (patho-) physiology3 .

保存條件:

The antibodies were purified from hybridoma cell culture supernatant by Protein G-Sepharose chromatography.

Stable for six months from date of shipment when stored at 4°C.

KEEP STERILE, the preparation contains no preservative. Aliquots can be stored at -20°C for at least one year. Avoid repeated freezing and thawing.

應用:

This preparation is optimized to reduce the platelet count by >95% within 60 minutes after intravenous injection of 2 µg/g bodyweight. It is recommended to inject the antibodies in a volume of 100-200 µl (diluted with sterile PBS).

同型對照(抗體來源): Rat IgG (Wistar)

緩沖液: 0.5 mg purified immunoglobulins in PBS, 0.2% (w/v) BSA, sterile filtered

濃度: 0.5 mg/ml

產品圖片:

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實驗結果:


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